Wednesday 7 December 2011

Adakah ini harapan bagi kita???

PD 404,182 is a virucidal small molecule that disrupts hepatitis C virus and human immunodeficiency virus

  1. Ana Maria Chamouna,
  2. Karuppiah Chockalingama,
  3. Michael Bobardtc,
  4. Rudo Simeona,
  5. Jinhong Changd,
  6. Philippe Gallayc,1 and
  7. Zhilei Chena,b,1
+ Author Affiliations
  1. aArtie McFerrin Department of Chemical Engineering, Texas A&M University, 3122 TAMU, College Station, TX 77843
  2. bDepartment of Microbial and Molecular Pathogenesis, Texas A&M Health Science Center, College Station, TX 77843
  3. cDepartment of Immunology and Microbial Sciences, IMM-9, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037
  4. dDrexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PA 18902

ABSTRACT

We describe a virucidal small molecule, PD 404,182, effective against hepatitis C virus (HCV) and human immunodeficiency virus (HIV). The median IC50 values for the antiviral effect of PD 404,182 against HCV and HIV in cell culture are 11 μM and 1 μM, respectively. The antiviral activity of PD 404,182 is due to physical disruption of virions that is accompanied to varying degrees (depending on the virus and exposure temperature/time) by release of viral nucleic acids into the surrounding medium. PD 404,182 does not directly lyse liposomal membranes even after extended exposure and shows no attenuation in antiviral activity when pre-incubated with liposomes of various lipid compositions, suggesting that the compound inactivates viruses through interaction with a non-lipid structural component of the virus. The virucidal activity of PD 404,182 appears to be virus-specific as little to no viral inactivation was detected with the enveloped Dengue and Sindbis viruses. PD 404,182 effectively inactivates a broad range of primary isolates of HIV-1 as well as HIV-2 and simian immunodeficiency virus (SIV), and does not exhibit significant cytotoxicity with multiple human cell lines in vitro (CC50 > 300 μM). The compound is fully active in cervical fluids although exhibiting decreased potency in the presence of human serum, retains its full antiviral potency for over 8 h when in contact with cells and is effective against both cell-free and cell-associated HIV. These qualities make PD 404,182 an attractive candidate microbicide for the prevention of HIV transmission through sexual intercourse.

8 comments:

  1. insyaAllah...
    pe2pun kekal berjuang dan teruskan perjuangan.

    kenapalah byk sgt energy ni InSaN@TaBaH?
    hehehehe

    ReplyDelete
  2. kalau laju camni, saya cadangkan InSaN@TaBaH publish 3,4 entry sekaligus. bimbang tak dibaca oleh org2 lain sbb semuanya bagus2.
    bkn semua rajin mencari dan klik sana sini.

    ReplyDelete
  3. Blog pun cantik.. cuma label banyak sangat. LAbel letak sigkat dan mudah contoh doa.. pengalaman. macam AHP buat hehe. Nak letak chat box ke? AO boleh bantu.. AO dulu un ada blog tapi AO delete sebab tak rajin nak update pastu AO rajin juga bantu kawan2 buat blog dulu.. hehe

    Apapun taniah! ini akan jadi antara blog kesukaan AO. dengar citer azam pun nak bukak blog.. boleh la AO baca hehe :)

    ReplyDelete
  4. AHP; biasalah insan tabah ni seorang yg baru je bangkit dari kedukaan. skrng dia tgh bersemangat nak teruskan khidupannya. alhamdulillah...

    huhuhu nak tau, insan tabah ni mudah berjurai air mata. puas dah azam bg tisu. sabar ya dik. be strong ok

    ReplyDelete
  5. AHP:Pada AHP terima kasih saya ucapkan atas segala dorongan...saya lebih suka melihat pada pencapaian dalam kajian berkaitan HIV...bukan apa secara tidak langsung kita tahu apa progres tentang HIV di dunia

    AZAM:saya memang macam tu, kuat diluar, tapi dalam kadang2 rapuh...

    AO:Terima kasih AO, saya mengalu2kan bantuan AO..dan sama2 juga kita bantu AZAM dengan BLOG barunya....

    ReplyDelete
  6. ok IT kalau ada apa2 nak tanya mesej Ao kat email ao yer

    ReplyDelete
  7. wah , wah jealousnya...semua org dah ad blog.

    ReplyDelete
  8. Abg Fareez x ada blog ke? Hehehehe

    ReplyDelete